Abstract:Bacterial flagella drive motility in many species, likely including the last bacterial common ancestor. Knowledge of flagellar assembly and function has mainly come from studies ofEscherichia coli and Salmonellaenterica, which have simple flagellar motors. However, most flagellated bacteria possess complex motors with unique, species-specific adaptations whose mechanisms and evolution remain largely unexplored. Here, we deploy a multidisciplinary approach to build a near-complete model of the flagellar motorin Campylobacter jejuni, revealing its remarkable complexity in architecture and composition. We identify an E-ring around the MS-ring, a periplasmic cage with two distinctive conformations, and an intricate interaction network between the E-ring and cage. These scaffolds play critical roles in stabilizing and regulating 17 torque-generating stator complexes for optimal motility.In-depth evolutionary analyses uncover the ancient origin and prevalence of the E-ring in flagellated species of the domain Bacteria as well as a unique exaptation of type IV pili components PilMNOPQF in the ancestral motor of the phylum Campylobacterota. Collectively, our studies reveal novel mechanisms of assembly and function in complex flagellar motors and shed light on the evolution of flagella and modern bacterial species.
个人简历：高贝乐，2003在山东大学获得生物学学士学位，2009在加拿大麦克马斯特大学获得生物化学和生物医学博士学位。2010-2014年，在美国耶鲁大学Dr. Jorge Galan实验室完成博士后研究。2014年起任中国科学院南海海洋研究所海洋微生物基因组学学科组组长。研究方向为细菌信号转导网络和进化基因组学。获得中科院百人计划、广东省杰出青年基金、广东省特支计划“青年拔尖人才”等人才项目资助。任中国微生物学会“普通微生物学专业委员会”和“分析微生物学专业委员会”委员（2021-2026），中国遗传学会“微生物遗传学专业委员会”委员（2024-2028）。美国微生物学会（ASM）期刊mBio和Microbiology Spectrum以及欧洲微生物学会联合会（FEMS）期刊FEMS Microbe和FEMS Infectious Disease编委。
主持人：吴法柏 理学部副教授
